Bio-rad SmartSpec Plus Spectrophotometer User Manual download pdf (Page 51)

7.6 Lambda

λλ

This assay allows you to collect absorbance data from up to three different wavelengths

simultaneously. No concentrations are calculated. When you press

λλ

, SmartSpec Plus will

prompt you first for the number of wavelengths to read.

• Use the number keys to input the number of wavelengths to be read and press

Enter. SmartSpec Plus will then ask for each individual wavelength.

• Use the number keys to input the wavelength and press Enter.

After all wavelengths are entered, choose if you want to subtract a background reading

from each wavelength. (The same background measurement is subtracted from all

readings.)

• If you do not want to subtract a background reading, press Enter and SmartSpec

Plus will be ready to collect absorbance data. If you want to subtract a background

reading, press Select to toggle from No to Yes and press Enter.

• Use the number keys to input the wavelength and press Enter.

SmartSpec Plus is now ready to collect absorbance data at the specified wavelengths.

For best results, you should first zero the SmartSpec Plus at specified wavelengths by

inserting a blank solution and pressing Read Blank.

Enter number (1–3) of

wavelengths to read: 3

Enter wavelength #1:

Do you want to subtract

a background reading? No

Enter wavelength for the

background reading:

Ready to read absorbance

<=Exit Assay >=Options

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Bio-rad SmartSpec Plus Spectrophotometer User Manual Page 51